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tartrate-resistant acid phosphatase (trap) buffer solution  (FUJIFILM)

 
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    FUJIFILM tartrate-resistant acid phosphatase (trap) buffer solution
    Tartrate Resistant Acid Phosphatase (Trap) Buffer Solution, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/trap+buffer+solution/tartrate+resistant+acid+phosphatase++trap++staining+kit/pm35584937-84-0-19
    Average 90 stars, based on 1 article reviews
    tartrate-resistant acid phosphatase (trap) buffer solution - by Bioz Stars, 2026-10
    90/100 stars

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    Staining:

    Article Title: The Effect of Methyl-β-cyclodextrin on the Differentiation of RAW264 Cells into Osteoclasts
    Article Snippet: with PBS(－), the cells were incubated for 4 days in a culture medium containing 50 ng/ml sRANKL and 0, 0.1, 0.5, or 1.0 mM of MβCD, respectively. .. TRAP buffer solution was prepared by mixing 0.1 M of sodium acetate (Wako Pure Chemicals, Osaka, Japan) and 50 mM of tartaric acid (Wako Pure Chemicals, Osaka, Japan) in a 1 :1 ratio, and adjusted to pH 5. .. TRAP staining solution was prepared by mixing 50 ml of TRAP buffer solution, 5 mg of naphthol AS-MX phosphate (SigmaAldrich, MO, USA), 0.5 ml of N,N-d



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    Identification of mature osteoclasts (mOC) on bone slices and quantification of resorbing activity 21 days after onset of the differentiation process. A <t>triple</t> <t>staining</t> for OC markers was applied <t>(TRAP,</t> red; F-actin, green; nuclei: blue; (A) . A cell was classified as mOC when it TRAP-positive and had ≥ 3 nuclei (white arrowheads; all mOC showed an actin ring, (B) , precursor (preOC, round with one or two nuclei, yellow arrowheads, (C) , proOC (spindle-shaped with a single nucleus, red arrowhead, (D) . After microscopy, cells were removed, and resorption pits were visualized by Toluidine blue staining (E) . Semi-quantitative image analysis was performed using ImageJ and masks to measure the resorbed areas per field of view (F) . Scale bar: 50 µm (A-D) , 100 µm (E, F) .
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    Identification of mature osteoclasts (mOC) on bone slices and quantification of resorbing activity 21 days after onset of the differentiation process. A <t>triple</t> <t>staining</t> for OC markers was applied <t>(TRAP,</t> red; F-actin, green; nuclei: blue; (A) . A cell was classified as mOC when it TRAP-positive and had ≥ 3 nuclei (white arrowheads; all mOC showed an actin ring, (B) , precursor (preOC, round with one or two nuclei, yellow arrowheads, (C) , proOC (spindle-shaped with a single nucleus, red arrowhead, (D) . After microscopy, cells were removed, and resorption pits were visualized by Toluidine blue staining (E) . Semi-quantitative image analysis was performed using ImageJ and masks to measure the resorbed areas per field of view (F) . Scale bar: 50 µm (A-D) , 100 µm (E, F) .
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    Average 90 stars, based on 1 article reviews
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    Identification of mature osteoclasts (mOC) on bone slices and quantification of resorbing activity 21 days after onset of the differentiation process. A triple staining for OC markers was applied (TRAP, red; F-actin, green; nuclei: blue; (A) . A cell was classified as mOC when it TRAP-positive and had ≥ 3 nuclei (white arrowheads; all mOC showed an actin ring, (B) , precursor (preOC, round with one or two nuclei, yellow arrowheads, (C) , proOC (spindle-shaped with a single nucleus, red arrowhead, (D) . After microscopy, cells were removed, and resorption pits were visualized by Toluidine blue staining (E) . Semi-quantitative image analysis was performed using ImageJ and masks to measure the resorbed areas per field of view (F) . Scale bar: 50 µm (A-D) , 100 µm (E, F) .

    Journal: Frontiers in Immunology

    Article Title: Modulation of Differentiation and Bone Resorbing Activity of Human (Pre-) Osteoclasts After X-Ray Exposure

    doi: 10.3389/fimmu.2022.817281

    Figure Lengend Snippet: Identification of mature osteoclasts (mOC) on bone slices and quantification of resorbing activity 21 days after onset of the differentiation process. A triple staining for OC markers was applied (TRAP, red; F-actin, green; nuclei: blue; (A) . A cell was classified as mOC when it TRAP-positive and had ≥ 3 nuclei (white arrowheads; all mOC showed an actin ring, (B) , precursor (preOC, round with one or two nuclei, yellow arrowheads, (C) , proOC (spindle-shaped with a single nucleus, red arrowhead, (D) . After microscopy, cells were removed, and resorption pits were visualized by Toluidine blue staining (E) . Semi-quantitative image analysis was performed using ImageJ and masks to measure the resorbed areas per field of view (F) . Scale bar: 50 µm (A-D) , 100 µm (E, F) .

    Article Snippet: The staining solution consists of TRAP buffer (0.1 M acetate buffer: 35.2 ml 0.2 M Sodium Acetate solution, 14.8 ml 0.2 M acetic acid, 50 ml; Millipore, Burlington, USA) mixed with 0.3 M Sodium Tartrate (pH 5.5), 10 mg/ml Naphthol AS-MX phosphate, Triton X-100, 0.3 mg/ml Fast Red Violet LB Salt (all from Sigma Aldrich Chemie GmbH).

    Techniques: Activity Assay, Staining, Microscopy